Abstract
Present research was intended to study the phytochemical, antioxidant
and antihyperglycemic activity of Phyllanthus emblica
fruits ethanolic extract (PEFEE). This involved the preparation of PEFEE
using cold maceration and soxhlet extraction, followed
by its phytochemical analysis and antihyperglycemic and antioxidant
activity. Maceration and soxhlet extraction of Phyllanthus
emblica using ethanol offered dark brown of Phyllanthus emblica fruits
ethanolic extract. The antihyperglycemic activity involved
STZ induced diabetic rats where they were treated with PEFEE at 200 mg
and 400 mg/kg body weight/day, or glibenclamide
(10 mg/kg body weight/day) for 21 days. Phytochemical screening of the
extract revealed the presence ophytoconstituents, such
as: carbohydrates, proteins, amino acids, steroids, flavonoids, tannins
and phenolic compounds, alkaloids, cardiac glycosides,
coumarins, anthraquinone, flavonoids and volatile oils The antioxidant
activity and total phenolic content wascarried out to identify
the IC50 value and the total amount of phenolic content of Phyllanthus
emblica. The DPPH radical scavenging assay and total
phenolic test were shown positive for the antioxidant activity of the
Phyllanthus emblica. As a basis, Folin-Ciocalteu reagent was
used to measure the phenolic content of different concentrations of
plant extract. In this test, the Gallic acid was used as a standard
and the total phenolic content was calculated by using the standard
formula In the present study, the total phenolic content found
was to be 6.420 mg GAE/g and 6.097 mg GAE/g for the concentrations of 50
and 200μg/ml respectively. According to the results
obtained, the IC50 was calculated for the BHT as well as Phyllanthus
emblica extract, the value was 84.04μg/ml and 182.82μg/ml,
respectively. However, it can be concluded that the Phyllanthus emblica
are exhibiting antioxidant effect as it shows positive results
for the total phenolic content (TPC) as well as DPPH scavenging method.
The antihyperglycemic activity revealed that Phyllanthus
emblica fruits extract has antihyperglycemic effect against STZ induced
diabetic rats and the extract maintains the BW of diabetic
rats. Present study established that the Phyllanthus emblica fruits
ethanolic extract possess antihyperglycemic activity.
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